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Title: | Purification and characterization of two acidic phospholipase A2 enzymes from king cobra (Ophiophagus hannah) snake venom | Authors: | Nget-Hong, T. Saifuddin, M.N. |
Issue Date: | 1990 | Journal: | Purification and characterization of two acidic phospholipase A2 enzymes from king cobra (Ophiophagus hannah) snake venom. International Journal of Biochemistry, 22(5), 481-487 | Abstract: | 1. 1. The two major phospholipase A2 enzymes (OHPLA-DE1 and OHPLA-DE2) of king cobra (Ophiophagus hannah) venom have been purified to electrophoretic homogeneity. 2. 2. The isoelectric points of OHPLA-DE1 and OHPLA-DE2 were 3.81 and 3.89, respectively and the Mws were 14,000 and 15,000, respectively, as estimated by Sephadex G-75 gel filtration chromatography; and 14,000 as estimated by SDS-PAGE. 3. 3. The enzymes were not lethal to mice at a dosage of 10 μg/g body wt by i.v. route. Both phospholipase A2 enzymes, however, exhibited moderate edema-inducing and anti-coagulant activities. 4. 4. Bromophenacylation of the enzymes reduced the enzymatic activity drastically but did not affect the edema-inducing activity of the enzymes. © 1990. | DOI: | 10.1016/0020-711X(90)90261-Z |
Appears in Collections: | COE Scholarly Publication |
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